The fluorescent azide is small enough to diffuse freely through native tissues and DNA, and it covalently cross-links to the EdU in a 'click' chemistry reaction.
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The assays … If incorrect, please enter your country/region into the box below, to view site information related to your country/region.Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.Access advice and support for any research roadblockFull event breakdown with abstracts, speakers, registration and moreGuaranteed product quality, expert customer support.EdU Assay / EdU Staining Proliferation Kit (iFluor 647) ab222421 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy. EdU Proliferation Assays. Unlike assays using BrdU, Click-iT EdU assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside.
Cells tagged with Click-iT EdU flow cytometry reagent can be measured against total DNA in a cell stained with Hoechst or PI (propidium iodide) or a specific labeled subset. 9 0 obj
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In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample.
HeLa cells (4 x 10 The left image shows proliferating cells labeled with EdU.
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This simplifies the assay considerably, and the results you achieve are similar to (or better than) those typically achieved using BrdU The Click-iT Plus formulation provides increased multiplexability compared to the original Click-iT EdU flow cytometry assays. ABP Biosciences is proud to introduce a series of iClick™ EdU Cell Proliferation Kits to detect Cell Proliferation in vitro and in vivo in a simple, two-step procedure.
A fluorescent azide, such as iFluor-488, is then added.
Faster Than BrdU assays - EdU detection requires less steps than is needed with antibody detection in the anti-BrdU assay, only requiring a total time of ~1 1/2 hr (30 min for the click ligation).
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Click-iT Plus EdU assays can be used in conjunction with R-PE and R-PE tandems, as well as fluorescent proteins such as GFP and mCherry, without loss of the accuracy or speed of the original Click-iT EdU assay.Not for resale.
Materials .
Reagent volumes may need to be optimized for other imaging geometries. Your browser does not have JavaScript enabled and some parts of this website will not work without it.For the best experience on the Abcam website please upgrade to a modern browser such as In a cell proliferation assay, the output should give you a direct and accurate measurement of the number of actively dividing cells in a population, be it cells in culture or tissues.
We use cookies to make our site as useful as possible. Add EdU …
The small size of the azide reagent allows efficient access to the DNA without the need for harsh cell treatment. The assay uses click chemistry to fluorescently detect the incorporation of EdU into newly synthesized DNA. Cell-division cycle is a series of events resulting in two daughter cells containing replicas of DNA from the original DNA molecule. 2.
The fluorescent azide is small enough to diffuse freely through … <>>>
EdU Assay / EdU Staining Proliferation Kit (iFluor 647)Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.Cross talk between 26S proteasome and mitochondria in human mesenchymal stem cells' ability to survive under hypoxia stress.Epithelial cells were grown with MEM medium supplement with 10%FBS. ",#(7),01444'9=82 The EdU cell proliferation assay does not require drastic DNA denaturation, it only needs the gentle cell immobilization and permeabilization to better protect the cell morphology, DNA structure and intracellular antigen recognition sites.
Submitted Mar 31 2020
EdU, Cell Proliferation Assay Cell proliferation assay with “click” chemistry as an alternative to 3H-thymidine and BrdU methods Product Description Name : 5-ethynyl-2'-deoxyuridine (EdU) Cell proliferation assay based on click chemistry Catalog Number : FP-MM9829, 25 mg FP-MM982B, 100 mg FP-MM982C, 500 mg Structure : C11H12N2O5 Click-&-Go EdU 405 Cell Proliferation Assay Kit Cat#: 1323 EdU cell proliferation assay optimized for fluorescence microscopy systems with 407 nm spectral line of …
The main advantages of EdU staining over using BrdU are:Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.Dot plot of EdU-647 staining (Y-axis, 647) vs FSC. <>
10EdU staining of proliferating cells. Half of the medium was replaced with EdU solution and cells were incubated 24h.
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It’s more suitable to multi-label with other dyes or protein labeling (eg: P65 antibody), directly access multi-dimensional cell features at …
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