The use of Invitrogen Alexa Fluor dyes results in a bright, photostable fluorescent signal designed for easy detection of proliferating cells.The Invitrogen Click-iT EdU Colorimetric IHC Detection Kit provides a simple, robust method for analyzing DNA replication in proliferating cells in as little as 90 minutes. Kits are available for microscopic imaging or HCS with a choice of fluorescent wavelengths for multiplexing.Invitrogen Click-iT EdU Assay Kits provide simple, robust methods for analyzing DNA replication in proliferating cells in as little as 90 minutes.
Proliferation measurements are typically made based on average DNA content or on cellular metabolism.
Not intended for any animal or human therapeutic or diagnostic use. into adult mice and tissues were harvested and fixed 96 h after injection. 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Assay kits are available for cultured cells or tissue sections analyzed using fluorescence microscopy, for HCS in microplates, or for microplate assays of cell lysates.The Click-iT EdU Microplate Assay is optimized for microplate-based fluorescence analysis with a simple and rapid workflow.In this assay the modified thymidine analog EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with bright, photostable Amplex UltraRed dye in a fast, highly specific click reaction. In this technique, the target antigen is the fluorescent azide (Oregon Green® 488 or tetramethylrhodamine (TAMRA™) dye) used to detect DNA that has incorporated EdU.
In addition to the hunt for more of the individual factors involved in DNA replication, the DNA synthesis assay allowed researchers to study the properties of DNA synthesis. Tracking New DNA Synthesis Our iClick™ EdU assays are designed to analyze the rate of new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA.
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